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Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments

Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments

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06:53 min

February 12, 2021

DOI:

06:53 min
February 12, 2021

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Summary

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Here, we described a protocol to quantitatively study the assembly and structure of the axon initial segments (AIS) of hippocampal neurons that lack pre-assembled AIS due to the absence of a giant ankyrin-G.

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