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Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
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Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

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05:22 min

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January 25, 2022

DOI:

10.3791/61407-v

DOI:
10.3791/61407-v

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05:22 min
January 25, 2022

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6 Views
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1Department of Biochemistry, Microbiology, and Immunology,University of Ottawa, 2Proteins Easy Corp

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This protocol allows for the preparation of transverse sections of cereal seeds (e.g., rice) for the analysis of endosperm and starch granule morphology using scanning electron microscopy.

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Demone, J., Barton, K. A., Altosaar, I. Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support. J. Vis. Exp. (179), e61407, doi:10.3791/61407 (2022).

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