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Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
JoVE Journal
Biochemie
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JoVE Journal Biochemie
Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

DOI:
10.3791/61407-v

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05:22 min

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January 25, 2022

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, ,
1Department of Biochemistry, Microbiology, and Immunology,University of Ottawa, 2Proteins Easy Corp

Kapitel

  • 00:04Introduction
  • 01:08Preparation of Transverse Rice Section
  • 02:33Reflected Light Microscopy and Scanning Electron Microscopy of Transverse Rice Sections
  • 02:56Results: SEM Images of Transverse Kernel Sections
  • 04:34Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol allows for the preparation of transverse sections of cereal seeds (e.g., rice) for the analysis of endosperm and starch granule morphology using scanning electron microscopy.

Tags

Transverse SectioningRice KernelScanning Electron MicroscopyPipette TipImmobilizationStarch GranulesResistant StarchAmylolysisGlycemic IndexReflected Light Microscopy

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