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Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
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JoVE Journal İmmünoloji ve Enfeksiyon
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

DOI:
10.3791/61359-v

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09:00 min

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June 24, 2020

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, , ,
1Department of Microbiology, Immunology, and Cell Biology,West Virginia University, 2Nörobilim Bölümü,West Virginia University, 3Rockefeller Neuroscience Institute,West Virginia University

Bölümler

  • 00:04Introduction
  • 00:45Lung Tissue Harvest
  • 02:50Lung Tissue Homogenization
  • 03:44Lung Tissue Dissociation and Single Cell Isolation
  • 05:21Flow Cytometric Lung Immune Cell Niche Analysis
  • 06:27Results: Representative Immune Cell Population and Chemokine Expression Characterization
  • 08:35Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol describes the use of flow cytometry to identify the changes in immune cell composition, cytokine profile, and chemokine profile in the pulmonary environment following transient middle cerebral artery occlusion, a murine model of ischemic stroke.

Etiketler

Immune CellsProinflammatory MediatorsPulmonary EnvironmentIschemic StrokeFlow Cytometric AnalysisLung Immune Cell TypesCytokinesChemokinesWhole Body Transcardial PerfusionMultiplex Bead ArraysHomogenization BufferLung Cell Medium

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