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Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
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Immunology and Infection
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JoVE Journal Immunology and Infection
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

English

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3,127 Views

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09:00 min

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June 24, 2020

DOI:

10.3791/61359-v

DOI:
10.3791/61359-v

•

09:00 min
June 24, 2020

•

1 Views
, , ,
1Department of Microbiology, Immunology, and Cell Biology,West Virginia University, 2Department of Neuroscience,West Virginia University, 3Rockefeller Neuroscience Institute,West Virginia University

Summary

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This protocol describes the use of flow cytometry to identify the changes in immune cell composition, cytokine profile, and chemokine profile in the pulmonary environment following transient middle cerebral artery occlusion, a murine model of ischemic stroke.

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Cite this Article

Monaghan, K. L., Farris, B. Y., Zheng, W., Wan, E. C. K. Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment. J. Vis. Exp. (160), e61359, doi:10.3791/61359 (2020).

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