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使用亚细胞室特异性红氧敏感绿色荧光蛋白评估细胞氧化
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Biyokimya
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JoVE Journal Biyokimya
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
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使用亚细胞室特异性红氧敏感绿色荧光蛋白评估细胞氧化

DOI:
10.3791/61229-v

•

06:10 min

•

June 18, 2020

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, , ,
1Division of Radiation Health, Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy,Ege University

Bölümler

  • 00:04Introduction
  • 00:46Cell Preparation
  • 01:33Adenoviral Redox Sensitive Green Fluorescent Protein (roGFP) Transduction
  • 02:46Cysteine/Cystine (CyS/CySS) Balance Acquisition
  • 04:01Results: Representative Cellular Oxidation Evaluation
  • 05:29Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

该协议描述了对细胞内亚细胞室特异性氧化还原状态的评估。对氧化还原敏感的荧光探针可在完整细胞中方便地进行比例分析。

Etiketler

Subcellular CompartmentRedox-sensitive GFPOxidative StressRedox StateRatiometricNon-disruptiveTransductionFlow CytometryHydrogen Peroxide

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