Dergi
/
İmmünoloji ve Enfeksiyon
/
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

DOI:
10.3791/61178-v

•

05:59 min

•

May 14, 2020

•

, ,
1Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University,Harbin Medical University, 2Inflammation Research Network, Department of Microbiology, Immunology and Infectious Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine,University of Calgary

Bölümler

  • 00:04Introduction
  • 00:40Preparation of Materials
  • 01:11Preparation of the Animal for Surgery
  • 01:47Surgical Suturing of Afferent Lymphatic Vessels
  • 03:47Tracking the Lymph Flow
  • 04:31Results: Selective Prevention of Lymph Flow
  • 05:09Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

A protocol to block lymph flow by surgical suturing of afferent lymphatic vessels is presented.

Etiketler

Lymph FlowLymphatic VesselsAfferent Lymphatic VesselsPopliteal Lymph NodesIntradermal InjectionEvans Blue DyeSutureMouseAnesthesiaMicrosurgery

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

Introducing Shear Stress in the Study of Bacterial Adhesion

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Read Article