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An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics,Medical University of Vienna (MUV), 2Murdoch Children’s Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health,Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry,Medical University of Vienna
Bölümler
- 00:04Introduction
- 00:35Preparing Solutions and Plates (Day 1)
- 01:50Blocking and Sample Addition (Day 2)
- 03:42Antibody Addition
- 05:17Reading the Plate
- 06:10Results: Accuracy and Consistency of MeCP2-ECLIA
- 07:20Conclusion
The electrochemiluminescence immunoassay (ECLIA) is a novel approach for quantitative detection of endogenous and exogenously applied MeCP2 protein variants, which produces highly quantitative, accurate and reproducible measurements with low intra- and inter-assay error over a wide working range. Here, the protocol for the MeCP2-ECLIA in a 96-well format is described.
