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An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
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07:44 min
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May 22, 2020
DOI:
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics,Medical University of Vienna (MUV), 2Murdoch Children’s Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health,Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry,Medical University of Vienna
Summary
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The electrochemiluminescence immunoassay (ECLIA) is a novel approach for quantitative detection of endogenous and exogenously applied MeCP2 protein variants, which produces highly quantitative, accurate and reproducible measurements with low intra- and inter-assay error over a wide working range. Here, the protocol for the MeCP2-ECLIA in a 96-well format is described.
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Steinkellner, H., Beribisky, A. V., Mausberg, P., Christodoulou, J., Scheiber-Mojdehkar, B., Huber, A., Sarne, V., Laccone, F. An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants. J. Vis. Exp. (159), e61054, doi:10.3791/61054 (2020).
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