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Delivery of Modified mRNA in a Myocardial Infarction Mouse Model
JoVE Journal
Genetik
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JoVE Journal Genetik
Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

DOI:
10.3791/60832-v

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06:03 min

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June 11, 2020

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, , , , , ,
1Cardiovascular Research Center,Icahn School of Medicine at Mount Sinai, 2Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai

'Bölümler'

  • 00:04Introduction
  • 01:06Preparation of modRNA Injection
  • 01:32Myocardial Infarction Surgery
  • 04:07Results: Validation of modRNA Delivery
  • 05:20Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

This protocol presents a simple and coherent way to transiently upregulate a gene of interest using modRNA after myocardial infarction in mice.

'Etiketler'

Modified RNAGene TransferMyocardial InfarctionIschemia ReperfusionTransverse Aortic ConstrictionIntratracheal IntubationLAD LigationIntramuscular Injection

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