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Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

DOI:

08:22 min

January 22, 2020

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Bölümler

  • 00:04Başlık
  • 00:32Sensor Cartridge and Extracellular Flux Analyzer Calibrant, Concanavalin A (ConA)-Coated Microplate, and Sperm Buffer Preparation
  • 01:52Cartridge Calibration Preparation and Wave Template Generation
  • 03:00Mouse Sperm Isolation
  • 03:42Sensor Cartridge Loading
  • 04:59Sperm Plate Preparation
  • 06:17Results: Representative Changes in Glycolysis and Oxidative Phosphorylation During Mouse Sperm Capacitation
  • 07:42Conclusion

Özet

Otomatik Çeviri

We describe the application of an extracellular flux analyzer to monitor real-time changes in glycolysis and oxidative phosphorylation during mouse sperm capacitation.

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