Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Verwendung eines extrazellulären Flux-Analyzers zur Messung von Veränderungen in der Glykolyse und oxidativen Phosphorylierung während der Maus Sperma-Capacitation
Bölümler
- 00:04Başlık
- 00:32Sensor Cartridge and Extracellular Flux Analyzer Calibrant, Concanavalin A (ConA)-Coated Microplate, and Sperm Buffer Preparation
- 01:52Cartridge Calibration Preparation and Wave Template Generation
- 03:00Mouse Sperm Isolation
- 03:42Sensor Cartridge Loading
- 04:59Sperm Plate Preparation
- 06:17Results: Representative Changes in Glycolysis and Oxidative Phosphorylation During Mouse Sperm Capacitation
- 07:42Conclusion
