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Genetik
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遺伝子組み換えマウスの高効率生産のための凍結解凍胚の使用
JoVE Journal
Genetik
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JoVE Journal
Genetik
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
Please note that all translations are automatically generated.
Click here for the English version.
遺伝子組み換えマウスの高効率生産のための凍結解凍胚の使用
DOI:
10.3791/60808-v
•
06:46 min
•
April 02, 2020
•
Hirofumi Nishizono*
1,2,3
,
Mohamed Darwish*
4,5
,
Hideki Uosaki
7
,
Nanami Masuyama
9,10
,
Motoaki Seki
11
,
Hiroyuki Abe
,
Nozomu Yachie
9,10,12,13
,
Ryohei Yasuda
1
Max Planck Florida Institute for Neuroscience
,
2
Life Science Research Center
,
University of Toyama
,
3
Department of Biochemical Engineering, Graduate School of Science and Engineering
,
Yamagata University
,
4
Graduate School of Innovative Life Science
,
University of Toyama
,
5
Department of Biochemistry, Faculty of Pharmacy
,
Cairo University
,
6
Division of Regenerative Medicine, Center for Molecular Medicine
,
Jichi Medical University
,
7
Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology
,
Jichi Medical University
,
8
Synthetic Biology Division, Research Center for Advanced Science and Technology
,
University of Tokyo
,
9
Institute for Advanced Biosciences
,
Keio University
,
10
Graduate School of Media and Governance
,
Keio University
,
11
Department of Molecular Oncology, Graduate School of Medicine
,
Chiba University
,
12
Department of Biological Sciences, School of Science
,
University of Tokyo
,
13
College of Arts and Sciences
,
University of Tokyo
'Bölümler'
00:05
Introduction
00:35
In Vitro Fertilization
02:23
Embryo Cryopreservation
03:13
Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Reagent Assembly
04:19
CRISPR Electroporation
05:09
Results: Representative Knockout Mouse Generation Efficiency and Mosaic Rate Evaluation
06:09
Conclusion
Özet
'Otomatik Çeviri'
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'Otomatik Çeviri'
ここでは、1細胞胚の凍結保存のための改変方法と、遺伝子組換えマウスの効率的な生成のための凍結解凍胚とエレクトロポレーションの使用を組み合わせたプロトコルを提示する。
'Etiketler'
Genetically Modified Mice
Freeze-thawed Embryos
In Vitro Fertilization
Super Ovulation
Sperm Capacitation
Cryopreservation
Embryo Electroporation
High-efficiency
Low Mosaic Rate
Protocol
Article
Embed
'ÇALMA LİSTESİNE EKLE'
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