ジャーナル
/
遺伝学
/
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
JoVE Journal
遺伝学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 遺伝学
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

DOI:
10.3791/60808-v

•

06:46 min

•

April 02, 2020

•

, , , , , , ,
1Max Planck Florida Institute for Neuroscience, 2Life Science Research Center,University of Toyama, 3Department of Biochemical Engineering, Graduate School of Science and Engineering,Yamagata University, 4Graduate School of Innovative Life Science,University of Toyama, 5Department of Biochemistry, Faculty of Pharmacy,Cairo University, 6Division of Regenerative Medicine, Center for Molecular Medicine,Jichi Medical University, 7Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology,Jichi Medical University, 8Synthetic Biology Division, Research Center for Advanced Science and Technology,東京大学, 9Institute for Advanced Biosciences,Keio University, 10Graduate School of Media and Governance,Keio University, 11Department of Molecular Oncology, Graduate School of Medicine,Chiba University, 12Department of Biological Sciences, School of Science,東京大学, 13College of Arts and Sciences,東京大学

章

  • 00:05Introduction
  • 00:35In Vitro Fertilization
  • 02:23Embryo Cryopreservation
  • 03:13Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Reagent Assembly
  • 04:19CRISPR Electroporation
  • 05:09Results: Representative Knockout Mouse Generation Efficiency and Mosaic Rate Evaluation
  • 06:09Conclusion

概要

自動翻訳

自動翻訳

Here, we present a modified method for cryopreservation of one-cell embryos as well as a protocol that couples the use of freeze-thawed embryos and electroporation for the efficient generation of genetically modified mice.

タグ

Genetically Modified MiceFreeze-thawed EmbryosIn Vitro FertilizationSuper OvulationSperm CapacitationCryopreservationEmbryo ElectroporationHigh-efficiencyLow Mosaic RateProtocol

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing

Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos

Production and Purification of Baculovirus for Gene Therapy Application

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii

Use of Drosophila S2 Cells for Live Imaging of Cell Division

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Read Article