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Визуализация и анализ фарингиальных архиерейных артерий с использованием иммуногистохимии и 3D-реконструкции
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JoVE Journal Gelişim Biyolojisi
Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction
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Визуализация и анализ фарингиальных архиерейных артерий с использованием иммуногистохимии и 3D-реконструкции

9,294 Views

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10:02 min

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March 31, 2020

DOI:

10.3791/60797-v

DOI:
10.3791/60797-v

•

10:02 min
March 31, 2020

•

1 Views
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1Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Özet

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Здесь мы описываем протокол для визуализации и анализа фарингиальных арочных артерий 3, 4 и 6 эмбрионов мыши с использованием иммунофлуоресценции, очистки тканей, конфокальной микроскопии и 3D-реконструкции.

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Cite this Article

Ramirez, A., Astrof, S. Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction. J. Vis. Exp. (157), e60797, doi:10.3791/60797 (2020).

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