Dergi
/
Gelişim Biyolojisi
/
Visualização e Análise de Artérias ariníngeos usando Imunohistoquímica de montagem integral e Reconstrução 3D
JoVE Journal
Gelişim Biyolojisi
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Gelişim Biyolojisi
Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

Visualização e Análise de Artérias ariníngeos usando Imunohistoquímica de montagem integral e Reconstrução 3D

9,294 Views

•

10:02 min

•

March 31, 2020

DOI:

10.3791/60797-v

DOI:
10.3791/60797-v

•

10:02 min
March 31, 2020

•

1 Views
,
1Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Özet

Automatically generated

Aqui, descrevemos um protocolo para visualizar e analisar as artérias do arco faríngeo 3, 4 e 6 dos embriões de camundongos usando imunofluorescência de montagem total, limpeza de tecidos, microscopia confocal e reconstrução 3D.

Explore More Videos

VisualizationAnalysisPharyngeal Arch ArteriesWhole-mount Immunohistochemistry3D ReconstructionEndothelial Cell QuantificationAortic Arch ArteriesCongenital Heart DiseaseVessel FormationRemodelingLight-sheet MicroscopyMouse EmbryoFixationPermeabilizationBlocking BufferPrimary AntibodySecondary AntibodyPlastic Paraffin Molds

Read Article

Cite this Article

Ramirez, A., Astrof, S. Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction. J. Vis. Exp. (157), e60797, doi:10.3791/60797 (2020).

Download .ris file

Copy

Share Video

.

Copy