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Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
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JoVE Journal İmmünoloji ve Enfeksiyon
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

7,468 Views

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09:34 min

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March 18, 2020

DOI:

10.3791/60680-v

DOI:
10.3791/60680-v

•

09:34 min
March 18, 2020

•

5 Views
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1Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP), 2Departamento de Ciências Biológicas, Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP)

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The overall goal of this protocol is to instruct how to extract, maintain, and dissociate murine astrocyte and microglia cells from the central nervous system, followed by infection with protozoa parasites.

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Pacheco, A. d. O. L., Amaral, M. P., de Farias, I. S., Bottino, L. Z. M. F., Bortoluci, K. R. Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection. J. Vis. Exp. (157), e60680, doi:10.3791/60680 (2020).

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