Dergi
/
İmmünoloji ve Enfeksiyon
/
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

DOI:
10.3791/60680-v

•

09:34 min

•

March 18, 2020

•

, , , ,
1Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP), 2Departamento de Ciências Biológicas, Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP)

Bölümler

  • 00:04Introduction
  • 01:31Glial Cells Extraction, Maintenance, and Dissociation
  • 07:28Results: Astrocytes and Microglia Infection
  • 08:32Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The overall goal of this protocol is to instruct how to extract, maintain, and dissociate murine astrocyte and microglia cells from the central nervous system, followed by infection with protozoa parasites.

Etiketler

Astrocyte IsolationMicroglia IsolationGlial Cell ExtractionCentral Nervous SystemImmunological ContextAlzheimer’sParkinson’sProtozoa ParasiteVirus Infection

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes

Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

Isolation, Transfection, and Culture of Primary Human Monocytes

Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA

Read Article