Journal
/
Immunologie et infection
/
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

7,477 Views

•

09:34 min

•

March 18, 2020

DOI:

10.3791/60680-v

DOI:
10.3791/60680-v

•

09:34 min
March 18, 2020

•

5 Views
, , , ,
1Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP), 2Departamento de Ciências Biológicas, Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP)

Summary

Automatically generated

The overall goal of this protocol is to instruct how to extract, maintain, and dissociate murine astrocyte and microglia cells from the central nervous system, followed by infection with protozoa parasites.

Explore More Videos

Astrocyte IsolationMicroglia IsolationGlial Cell ExtractionCentral Nervous SystemImmunological ContextAlzheimer’sParkinson’sProtozoa ParasiteVirus Infection

Read Article

Cite this Article

Pacheco, A. d. O. L., Amaral, M. P., de Farias, I. S., Bottino, L. Z. M. F., Bortoluci, K. R. Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection. J. Vis. Exp. (157), e60680, doi:10.3791/60680 (2020).

Download .ris file

Copy

Share Video

.

Copy