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将激光捕获微切片和微流体qPCR相结合，分析单细胞的转录曲线：阿片类药物依赖的系统生物学方法
JoVE Journal
Genetik
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JoVE Journal Genetik
Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence
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将激光捕获微切片和微流体qPCR相结合，分析单细胞的转录曲线：阿片类药物依赖的系统生物学方法

DOI:
10.3791/60612-v

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09:54 min

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March 08, 2020

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, , , ,
1Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology,Thomas Jefferson University, 2Sidney Kimmel Medical College,Thomas Jefferson University, 3Department of Pharmacology & Physiology,Drexel University College of Medicine

Bölümler

  • 00:05Introduction
  • 00:59Preparing the Samples
  • 02:16Laser Capture Microdissection
  • 04:07Single-cell Microfluidic RT-qPCR
  • 08:08Results: Microfluidic qPCR Yields Accurate and Anatomically Specific Transcriptional Profiles of Single-Cells
  • 09:21Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

该协议解释了如何使用激光捕获微解剖，以高精度和解剖特异性从杏仁核的中央核收集单个神经元、微胶质和星形细胞。此外，我们解释我们使用微流体RT-qPCR测量这些细胞转录的子集。

Etiketler

Laser Capture MicrodissectionMicrofluidic QPCRTranscriptional ProfilesSingle CellsSystems BiologyOpioid DependenceGene ExpressionCellular SubphenotypesAnatomic SpecificityBiological ProblemsNeuronsBrain RegionsAmygdalaEthanol DehydrationXyleneFluorescenceLCM SoftwareInfrared Laser

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