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Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence
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March 08, 2020
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This protocol explains how to collect single neurons, microglia, and astrocytes from the central nucleus of the amygdala with high accuracy and anatomic specificity using laser capture microdissection. Additionally, we explain our use of microfluidic RT-qPCR to measure a subset of the transcriptome of these cells.
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O'Sullivan, S. J., Reyes, B. A., Vadigepalli, R., Van Bockstaele, E. J., Schwaber, J. S. Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence. J. Vis. Exp. (157), e60612, doi:10.3791/60612 (2020).
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