This content is Free Access.
通过基因表达分析的改良方法从谷物种子获取高质量的转录数据
7,096 Views
•
07:18 min
•
May 21, 2020
DOI:
1Department of Chemistry and Biotechnology,Swinburne University of Technology, 2Department of Molecular Genetics, Heterosis Research Group,Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 3Grain Quality and Nutrition Center, Applied Functional Genomics Cluster,International Rice Research Institute
Özet
Automatically generated
提出了谷物转录分析的方法。基于微阵列的基因表达分析从谷物中分离高质量的总RNA开始，并随着cDNA的生成而继续。在cRNA标签和微阵列杂交后，提出了信号检测和质量控制的建议。
Read Article
Cite this Article
Butardo Jr., V. M., Seiler, C., Sreenivasulu, N., Kuhlmann, M. Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling. J. Vis. Exp. (159), e60602, doi:10.3791/60602 (2020).
Copy