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A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
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April 30, 2020
DOI:
1Division of Infection and Global Health, School of Medicine,University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences,University of Glasgow
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We describe a tuberculosis molecular bacterial load assay test performed after heat inactivation of sputum. Heat inactivation renders sputum samples noninfectious and obviates the need for containment level 3 laboratories for tuberculosis molecular tests.
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Sabiiti, W., Mtafya, B., De Lima, D. A., Dombay, E., Baron, V. O., Azam, K., Oravcova, K., Sloan, D. J., Gillespie, S. H. A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA). J. Vis. Exp. (158), e60460, doi:10.3791/60460 (2020).
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