Dergi
/
İmmünoloji ve Enfeksiyon
/
A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
JoVE Journal
İmmünoloji ve Enfeksiyon
This content is Free Access.
JoVE Journal İmmünoloji ve Enfeksiyon
A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)

A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)

DOI:
10.3791/60460-v

•

10:41 min

•

April 30, 2020

•

, , , , , , , ,
1Division of Infection and Global Health, School of Medicine,University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences,University of Glasgow

Bölümler

  • 00:05Introduction
  • 01:33Sample Preparation and Heat Inactivation
  • 02:30RNA Extraction
  • 06:28Reverse Transcriptase qPCR
  • 07:57Results: Heat Inactivation of M. tuberculosis Bacilli
  • 09:43Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We describe a tuberculosis molecular bacterial load assay test performed after heat inactivation of sputum. Heat inactivation renders sputum samples noninfectious and obviates the need for containment level 3 laboratories for tuberculosis molecular tests.

Etiketler

Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)TuberculosisDisease BurdenAnti-tuberculosis MedicationTreatment ResponseQuantitative ResultNontuberculous MycobacteriaChronic Obstructive Pulmonary DiseaseBacille Calmette-Guerin (BCG)Sputum SamplesRNA ExtractionPhenol ChloroformExtraction Control

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Using Luciferase to Image Bacterial Infections in Mice

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Growth of Mycobacterium tuberculosis Biofilms

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Read Article