This content is Free Access.
A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
13,321 Views
•
10:41 min
•
April 30, 2020
DOI:
1Division of Infection and Global Health, School of Medicine,University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences,University of Glasgow
Summary
Automatically generated
We describe a tuberculosis molecular bacterial load assay test performed after heat inactivation of sputum. Heat inactivation renders sputum samples noninfectious and obviates the need for containment level 3 laboratories for tuberculosis molecular tests.
Explore More Videos
Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)TuberculosisDisease BurdenAnti-tuberculosis MedicationTreatment ResponseQuantitative ResultNontuberculous MycobacteriaChronic Obstructive Pulmonary DiseaseBacille Calmette-Guerin (BCG)Sputum SamplesRNA ExtractionPhenol ChloroformExtraction Control
Read Article
Cite this Article
Sabiiti, W., Mtafya, B., De Lima, D. A., Dombay, E., Baron, V. O., Azam, K., Oravcova, K., Sloan, D. J., Gillespie, S. H. A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA). J. Vis. Exp. (158), e60460, doi:10.3791/60460 (2020).
Copy