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Выражение, очищение и липосома Связывание подающих надежды дрожжей SNX-BAR Heterodimers
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JoVE Journal Genetik
Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
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Выражение, очищение и липосома Связывание подающих надежды дрожжей SNX-BAR Heterodimers

7,576 Views

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10:28 min

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December 06, 2019

DOI:

10.3791/60413-v

DOI:
10.3791/60413-v

•

10:28 min
December 06, 2019

•

2 Views
, , ,
1Department of Cell Biology,Yale School of Medicine, 2Department of Biological Sciences,University of North Carolina at Charlotte

Özet

Automatically generated

Здесь мы представляем рабочий процесс для выражения, очищения и липосомы связывания гетеродимеров SNX-BAR в дрожжах.

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SNX-BARMembrane RemodelingLiposome BindingYeast ExpressionProtein PurificationIgG Sepharose BeadsCell LysisCentrifugationExtrusionBiophysical PropertiesLipid Specificity

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Ma, M., Goyal, S., Burd, C. G., Chi, R. J. Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers. J. Vis. Exp. (154), e60413, doi:10.3791/60413 (2019).

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