This protocol was generated as a means to produce brain organoids in a simplified, low cost manner without exogenous growth factors or basement membrane matrix while still maintaining the diversity of brain cell types and many features of cellular organization.
Boisvert, E. M., Means, R. E., Michaud, M., Thomson, J. J., Madri, J. A., Katz, S. G. A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells. J. Vis. Exp. (157), e60379, doi:10.3791/60379 (2020).