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A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
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JoVE Revista Biología del desarrollo
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

DOI:
10.3791/60379-v

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08:30 min

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March 04, 2020

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, , , , ,
1Department of Pathology,Yale University School of Medicine, 2Stem Cell Center,Yale University School of Medicine

Capítulos

  • 00:05Introduction
  • 00:53Stem Cell Maintenance
  • 03:03Dissociation of the hESCs for Organoid Culture
  • 04:27Generation of Organoids
  • 06:06Results: Overview of Organoid Growth Conditions and Morphology
  • 07:37Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol was generated as a means to produce brain organoids in a simplified, low cost manner without exogenous growth factors or basement membrane matrix while still maintaining the diversity of brain cell types and many features of cellular organization.

Tags

Brain OrganoidsHuman Embryonic Stem CellsNeurodevelopmental DisordersToxic InsultsBrain CancerReproducibleSimple WorkflowCost-effectiveMatrixH9 HESCsMTeSR1 MediaLow Oxygen IncubatorProtease SolutionPassaging

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