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Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

6,190 Views

09:34 min

October 31, 2019

DOI:

09:34 min
October 31, 2019

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Özet

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This study describes the microscopic monitoring of pneumococcus adherence to von Willebrand factor strings produced on the surface of differentiated human primary endothelial cells under shear stress in defined flow conditions. This protocol can be extended to detailed visualization of specific cell structures and quantification of bacteria by applying differential immunostaining procedures.

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