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Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

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09:34 min

October 31, 2019

DOI:

09:34 min
October 31, 2019

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Summary

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This study describes the microscopic monitoring of pneumococcus adherence to von Willebrand factor strings produced on the surface of differentiated human primary endothelial cells under shear stress in defined flow conditions. This protocol can be extended to detailed visualization of specific cell structures and quantification of bacteria by applying differential immunostaining procedures.

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