Presented here is a protocol for enriching pachytene spermatocytes, round spermatids, and elongating spermatids from adult mouse testes using a discontinuous bovine serum albumin density gradient with standard laboratory equipment.
Da Ros, M., Lehtiniemi, T., Olotu, O., Meikar, O., Kotaja, N. Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment. J. Vis. Exp. (151), e60271, doi:10.3791/60271 (2019).