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Journal / Developmental Biology / Enrichment of Pachytene Spermatocytes and…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
 

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Article DOI: 10.3791/60271-v • 10:22 min • September 17th, 2019
September 17th, 2019 •
*1, *1, 1, 1, 1
1Institute of Biomedicine, University of Turku
* These authors contributed equally

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Resumo
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Presented here is a protocol for enriching pachytene spermatocytes, round spermatids, and elongating spermatids from adult mouse testes using a discontinuous bovine serum albumin density gradient with standard laboratory equipment.

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MDR Method Pachytene Spermatocytes Round Spermatids Elongating Spermatids Mouse Testes Laboratory Equipment Functional Studies Male Germ Cells Male Fertility Paternal Epigenetic Inheritance Starting Material Water Bath Cell Culture Incubator Tube Rotator Microscopy Glass Slides Grease Pen Ventral Abdomen 70% Ethanol
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