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成年斑马鱼端脑中疟原虫DNA在活体传递的电穿孔方法
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JoVE Journal Gelişim Biyolojisi
Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon
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成年斑马鱼端脑中疟原虫DNA在活体传递的电穿孔方法

9,439 Views

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06:02 min

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September 13, 2019

DOI:

10.3791/60066-v

DOI:
10.3791/60066-v

•

06:02 min
September 13, 2019

•

4 Views
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1Institute of Stem Cell Research,Helmholtz Zentrum München, 2Graduate School of Systemic Neurosciences,Ludwig Maximilian University, 3Department of Cell Biology and Anatomy, Biomedical Center,Ludwig Maximilian University, 4Munich Cluster for Systems Neurology (SyNergy)

Özet

Automatically generated

这里介绍的是一种在成年斑马鱼端脑中进行质粒DNA传递和脑细胞标记的电穿孔方法。该协议是一种快速而有效的方法，用于可视化和跟踪单个细胞，并开辟了将电穿孔应用于各种基因操作的新的可能性。

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ElectroporationPlasmid DNAIn Vivo DeliveryZebrafish TelencephalonEpendymoglial CellsCell LabelingCell-autonomous ManipulationCell BiologyCell DelaminationEpithelial HomeostasisCell Division SymmetryGlass CapillaryMicroinjectionAnesthesiaStereomicroscopeMicroknifeTelencephalic VentricleUltrasound GelElectroporation

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Durovic, T., Ninkovic, J. Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon. J. Vis. Exp. (151), e60066, doi:10.3791/60066 (2019).

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