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Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon

Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon

DOI:
10.3791/60066-v

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06:02 min

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September 13, 2019

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,
1Institute of Stem Cell Research,Helmholtz Zentrum München, 2Graduate School of Systemic Neurosciences,Ludwig Maximilian University, 3Department of Cell Biology and Anatomy, Biomedical Center,Ludwig Maximilian University, 4Munich Cluster for Systems Neurology (SyNergy)

Bölümler

  • 00:04Başlık
  • 00:50Injection Setup
  • 01:33Plasmid Solution Injection
  • 02:47Electroporation
  • 03:30Results: Representative Ependymoglial Cell Electroporation Outcomes
  • 05:21Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Presented here is an electroporation method for plasmid DNA delivery and ependymoglial cell labeling in the adult zebrafish telencephalon. This protocol is a quick and efficient method to visualize and trace individual ependymoglial cells and opens up new possibilities to apply electroporation to a broad range of genetic manipulations.

Etiketler

ElectroporationPlasmid DNAIn Vivo DeliveryZebrafish TelencephalonEpendymoglial CellsCell LabelingCell-autonomous ManipulationCell BiologyCell DelaminationEpithelial HomeostasisCell Division SymmetryGlass CapillaryMicroinjectionAnesthesiaStereomicroscopeMicroknifeTelencephalic VentricleUltrasound GelElectroporation

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