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外迁移系统中淋巴细胞迁移的评估
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JoVE Journal İmmünoloji ve Enfeksiyon
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
Please note that all translations are automatically generated. Click here for the English version.

外迁移系统中淋巴细胞迁移的评估

6,639 Views

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10:25 min

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September 20, 2019

DOI:

10.3791/60060-v

DOI:
10.3791/60060-v

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10:25 min
September 20, 2019

•

1 Views
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1İç Hastalıkları Anabilim Dalı,Utah Üniversitesi, 2Research Service,VA Nebraska Iowa Health Care System, 3İç Hastalıkları Anabilim Dalı,University of Nebraska Medical Center, 4Department of Environmental, Agricultural & Occupational Health,University of Nebraska Medical Center

Özet

Automatically generated

在此协议中，淋巴细胞被放置在迁移系统的顶室中，由多孔膜与底部腔室隔开。化学因子被添加到底部腔室，从而诱导沿化学基质梯度的活性迁移。48小时后，淋巴细胞在两个腔室计数，以定量迁移。

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Cite this Article

Warren, K. J., Wyatt, T. A. Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System. J. Vis. Exp. (151), e60060, doi:10.3791/60060 (2019).

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