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Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs
JoVE Journal
Genetik
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JoVE Journal Genetik
Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs

Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs

7,397 Views

08:49 min

September 16, 2019

DOI:

08:49 min
September 16, 2019

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Özet

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We present a detailed small RNA library reparation protocol with less bias than standard methods and an increased sensitivity for 2'-O-methyl RNAs. This protocol can be followed using homemade reagents to save cost or using kits for convenience.

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