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Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs
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JoVE Journal Genetik
Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs

Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs

DOI:
10.3791/60056-v

•

08:49 min

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September 16, 2019

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, ,
1Institute for Integrative Biology of the Cell, UMR9198, CNRS CEA Univ Paris-Sud,Université Paris-Saclay

Kapitel

  • 00:04Titel
  • 00:33Small RNA Isolation
  • 02:26Unligated 3’ Adapter Elimination
  • 04:41Gel Purification
  • 05:27Raw Sequence File Treatment
  • 06:06Trimmed Sequence Mapping
  • 06:58Results: Representative 2’-OMe RNA Detection
  • 08:18Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We present a detailed small RNA library reparation protocol with less bias than standard methods and an increased sensitivity for 2'-O-methyl RNAs. This protocol can be followed using homemade reagents to save cost or using kits for convenience.

Tags

Small RNA-seqRNA Detection2′-O-Methyl RNAsUnbiased MethodsPlant MicroRNAsGel ElectrophoresisRNA ExtractionAdapter EliminationMagnetic Purification Beads

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