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Aislamiento, transfección y cultivo de monocitos humanos primarios
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolation, Transfection, and Culture of Primary Human Monocytes
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Aislamiento, transfección y cultivo de monocitos humanos primarios

12,768 Views

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09:13 min

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December 16, 2019

DOI:

10.3791/59967-v

DOI:
10.3791/59967-v

•

09:13 min
December 16, 2019

•

17 Views
, , , , ,
1Tıp Fakültesi,Louisiana State University Health Sciences Center, 2Department of Microbiology, Immunology, and Parasitology,Louisiana State University Health Sciences Center, 3Department of Biomedical and Clinical Sciences L. Sacco,University of Milan

Özet

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Aquí se presenta un protocolo optimizado para aislar, cultivar, transfectar y diferenciar a los monocitos primarios humanos de individuos infectados por el VIH y controles saludables.

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Plaisance-Bonstaff, K., Faia, C., Wyczechowska, D., Jeansonne, D., Vittori, C., Peruzzi, F. Isolation, Transfection, and Culture of Primary Human Monocytes. J. Vis. Exp. (154), e59967, doi:10.3791/59967 (2019).

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