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原人类单核细胞的隔离、转染和文化
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolation, Transfection, and Culture of Primary Human Monocytes
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原人类单核细胞的隔离、转染和文化

12,768 Views

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09:13 min

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December 16, 2019

DOI:

10.3791/59967-v

DOI:
10.3791/59967-v

•

09:13 min
December 16, 2019

•

17 Views
, , , , ,
1Tıp Fakültesi,Louisiana State University Health Sciences Center, 2Department of Microbiology, Immunology, and Parasitology,Louisiana State University Health Sciences Center, 3Department of Biomedical and Clinical Sciences L. Sacco,University of Milan

Özet

Automatically generated

这里介绍的是一个优化的协议，用于分离、培养、转染和区分人类原发性单核细胞与艾滋病毒感染者和健康对照。

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Cite this Article

Plaisance-Bonstaff, K., Faia, C., Wyczechowska, D., Jeansonne, D., Vittori, C., Peruzzi, F. Isolation, Transfection, and Culture of Primary Human Monocytes. J. Vis. Exp. (154), e59967, doi:10.3791/59967 (2019).

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