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Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
JoVE Journal
Gelişim Biyolojisi
This content is Free Access.
JoVE Journal Gelişim Biyolojisi
Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

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06:57 min

July 20, 2019

DOI:

06:57 min
July 20, 2019

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This protocol describes an approach to produce hepatospheres from human pluripotent stem cells using a defined culture system and cell self-assembly. This protocol is reproducible in a number of cell lines, cost effective and allows the production of stable human hepatospheres for biomedical application.

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