/
/
Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
This content is Free Access.
Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
8,509 Views
•
06:57 min
•
July 20, 2019
DOI:
1MRC Centre for Regenerative Medicine,University of Edinburgh, 2UCL Great Ormond Street Institute of Child Health,University College London, 3Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences,Brunel University London, 4School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute,Trinity College Dublin
Summary
Automatically generated
This protocol describes an approach to produce hepatospheres from human pluripotent stem cells using a defined culture system and cell self-assembly. This protocol is reproducible in a number of cell lines, cost effective and allows the production of stable human hepatospheres for biomedical application.
Read Article
Cite this Article
Lucendo-Villarin, B., Rashidi, H., Alhaque, S., Fischer, L., Meseguer-Ripolles, J., Wang, Y., O'Farrelly, C., Themis, M., Hay, D. C. Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells. J. Vis. Exp. (149), e59965, doi:10.3791/59965 (2019).
Copy