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Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
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JoVE Journal Biyokimya
Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

DOI:
10.3791/59883-v

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00:15 min

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September 28, 2019

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, , , , , ,
1Department of Biological Sciences,Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45137-66731, Iran

Bölümler

  • 00:00Başlık
  • 01:10Brain Homogenization and Mitochondrial Isolation
  • 04:33Mitochondrial Membrane Integrity Determination
  • 05:58Malate Dehydrogenase Activity Determination
  • 07:07In vitro α-synuclein, Bovine Insulin, and HEWL Fibril Formation
  • 10:11Treatment of Mitochondria with Amyloid Fibrils, MDH Release Assay, and ROS Measurement
  • 12:14Sonuçlar
  • 14:07Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Provided here is a protocol for investigating the interactions between native form, prefibrillar, and mature amyloid fibrils of different peptides and proteins with mitochondria isolated from different tissues and various areas of the brain.

Etiketler

MitochondriaAmyloid FibrilsAlpha-synucleinMembrane PermeabilizationNeurodegenerative DiseasesBrainIsolationHomogenizationCentrifugationDensity GradientMyelinMitochondrial Fraction

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