Provided here is a protocol for investigating the interactions between native form, prefibrillar, and mature amyloid fibrils of different peptides and proteins with mitochondria isolated from different tissues and various areas of the brain.
Zadali, R., Ghareghozloo, E. R., Ramezani, M., Hassani, V., Rafiei, Y., Chiyaneh, S. M., Meratan, A. A. Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils. J. Vis. Exp. (151), e59883, doi:10.3791/59883 (2019).