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Tuning Degradation to Achieve Specific and Efficient Protein Depletion
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Tuning Degradation to Achieve Specific and Efficient Protein Depletion
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July 20, 2019
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Here, we present a protocol to effectively and specifically deplete a protein of interest in the yeast Saccharomyces cerevisiae using the β-est AID system.
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Barrass, J. D., Mendoza-Ochoa, G. I., Maudlin, I. E., Sani, E., Beggs, J. D. Tuning Degradation to Achieve Specific and Efficient Protein Depletion. J. Vis. Exp. (149), e59874, doi:10.3791/59874 (2019).
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