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In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo
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In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster In Vivo
Bölümler
- 00:04Başlık
- 00:46Protocol 1: Climbing Assay
- 01:41Protocol 3: Histology Analysis
- 04:32Protocol 4: Gene Mapping of Recessive Mutant Phenotype
- 06:17Protocol 5: DNA Sequencing and Analysis
- 07:08Protocol 6: Further Analysis of Candidate Gene Function
- 07:46Results: Climbing Pass Rate, Histological Screen, and Deficiency Mapping
- 09:11Conclusion
Nous présentons un protocole utilisant une approche génétique avancée au dépiste pour des mutants présentant la neurodégénérescence dans le melanogaster de Drosophila. Il intègre un test d'escalade, l'analyse histologique, la cartographie des gènes et le séquençage de l'ADN pour finalement identifier de nouveaux gènes liés au processus de neuroprotection.
