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In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster
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JoVE Journal Genetik
In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

DOI:
10.3791/59720-v

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10:00 min

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July 11, 2019

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, , , , , , , , ,
1Department of Biological Sciences,University of Alabama, 2Genetik Bölümü,University of Wisconsin-Madison

Bölümler

  • 00:04Başlık
  • 00:46Protocol 1: Climbing Assay
  • 01:41Protocol 3: Histology Analysis
  • 04:32Protocol 4: Gene Mapping of Recessive Mutant Phenotype
  • 06:17Protocol 5: DNA Sequencing and Analysis
  • 07:08Protocol 6: Further Analysis of Candidate Gene Function
  • 07:46Results: Climbing Pass Rate, Histological Screen, and Deficiency Mapping
  • 09:11Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We present a protocol using a forward genetic approach to screen for mutants exhibiting neurodegeneration in Drosophila melanogaster. It incorporates a climbing assay, histology analysis, gene mapping and DNA sequencing to ultimately identify novel genes related to the process of neuroprotection.

Etiketler

Forward Genetic ScreenDrosophila MelanogasterNeuroprotective GenesNeurodegenerationAlzheimer’s DiseaseParkinson’s DiseaseNeuronal FunctionENU MutagenesisFly LocomotionCarnoy’s FixativeParaffin Embedding

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