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In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster
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JoVE Journal Genetik
In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

9,139 Views

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10:00 min

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July 11, 2019

DOI:

10.3791/59720-v

DOI:
10.3791/59720-v

•

10:00 min
July 11, 2019

•

8 Views
, , , , , , , , ,
1Department of Biological Sciences,University of Alabama, 2Genetik Bölümü,University of Wisconsin-Madison

Özet

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We present a protocol using a forward genetic approach to screen for mutants exhibiting neurodegeneration in Drosophila melanogaster. It incorporates a climbing assay, histology analysis, gene mapping and DNA sequencing to ultimately identify novel genes related to the process of neuroprotection.

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Cite this Article

Gevedon, O., Bolus, H., Lye, S. H., Schmitz, K., Fuentes-González, J., Hatchell, K., Bley, L., Pienaar, J., Loewen, C., Chtarbanova, S. In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster. J. Vis. Exp. (149), e59720, doi:10.3791/59720 (2019).

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