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末梢単核細胞からの誘導神経幹細胞の生成と移植研究におけるドーパミン作動性ニューロン前駆体への分化
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
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末梢単核細胞からの誘導神経幹細胞の生成と移植研究におけるドーパミン作動性ニューロン前駆体への分化

6,955 Views

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12:13 min

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July 11, 2019

DOI:

10.3791/59690-v

DOI:
10.3791/59690-v

•

12:13 min
July 11, 2019

•

3 Views
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1Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration,Ministry of Education, 2Center of Neural Injury and Repair,Beijing Institute for Brain Disorders

Özet

Automatically generated

このプロトコルは、仙台ウイルス感染による神経幹細胞を誘導する末梢血単核細胞のリプログラミング、ドーパミン作動性ニューロンへのiNSCの分化、DA前駆体の一方的な病変への移植を提示するパーキンソン病マウスモデル、およびPD治療のためのiNSC由来DA前駆体の安全性および有効性の評価。

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Zheng, W., Chen, Z. Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies. J. Vis. Exp. (149), e59690, doi:10.3791/59690 (2019).

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