Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Wei Zheng; Zhiguo Chen

doi:10.3791/59690

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Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

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発生生物学

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JoVE Journal 発生生物学

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

6,963 Views

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12:13 min

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July 11, 2019

DOI:

10.3791/59690-v

DOI:

10.3791/59690-v

•

12:13 min

July 11, 2019

•

3 Views

Wei Zheng^1,2, Zhiguo Chen^1,2

¹Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration,Ministry of Education, ²Center of Neural Injury and Repair,Beijing Institute for Brain Disorders

概要

The protocol presents the reprogramming of peripheral blood mononuclear cells to induce neural stem cells by Sendai virus infection, differentiation of iNSCs into dopaminergic neurons, transplantation of DA precursors into the unilaterally-lesioned Parkinson's disease mouse models, and evaluation of the safety and efficacy of iNSC-derived DA precursors for PD treatment.

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Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

概要

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