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Developmental Biology

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JoVE Journal Developmental Biology
Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
 

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Article DOI: 10.3791/59690-v • 12:13 min • July 11th, 2019
July 11th, 2019 •
1,2, 1,2
1Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, 2Center of Neural Injury and Repair, Beijing Institute for Brain Disorders

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The protocol presents the reprogramming of peripheral blood mononuclear cells to induce neural stem cells by Sendai virus infection, differentiation of iNSCs into dopaminergic neurons, transplantation of DA precursors into the unilaterally-lesioned Parkinson's disease mouse models, and evaluation of the safety and efficacy of iNSC-derived DA precursors for PD treatment.

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Induced Neural Stem Cells Peripheral Mononuclear Cells Neurodegenerative Diseases Parkinson's Disease Autologous Cell Source Induced Pluripotent Stem Cells Proliferative Capacity Region-specific Neural Cells Spinal Cord Neurons Motor Neurons Treatment Of Neurological Diseases Visual Demonstration Successful Generation Of Desired Cells Peripheral Venous Blood Dulbecco's PBS Density Gradient Medium
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