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Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer’s Disease
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Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer’s Disease

Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer’s Disease

8,889 Views

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06:52 min

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July 06, 2019

DOI:

10.3791/59597-v

DOI:
10.3791/59597-v

•

06:52 min
July 06, 2019

•

4 Views
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1Division of Pharmacy and Optometry, School of Health Studies, Faculty of Biology, Medicine and Health,University of Manchester, 2Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health,University of Manchester

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This protocol illustrates a cell encapsulation method by rapid physical gelation of alginate to immobilize cells. Obtained microbeads allow controlled and sustained secretion of amyloid-β over time and can be used to study the effects of secreted amyloid-β in in vitro and in vivo models.

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Almari, B., Brough, D., Harte, M., Tirella, A. Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer's Disease. J. Vis. Exp. (149), e59597, doi:10.3791/59597 (2019).

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