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June 16, 2019
DOI:
1Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, 2Department of Biological Sciences,Notre Dame Üniversitesi, 3Department of Urology, Shanghai East Hospital,Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases,Notre Dame Üniversitesi, 5Harper Cancer Research Institute,Notre Dame Üniversitesi, 6Tumor Microenvironment and Metastasis Program,Indiana University Melvin and Bren Simon Cancer Center
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由于三硝基酪氨酸修饰的丰度较低,酪氨酸硝酸蛋白的蛋白组谱分析一直是一项具有挑战性的技术。在这里,我们描述了一种利用血管紧张蛋白II作为模型来增强和分析硝基肽扩脂的新方法。此方法可以扩展到其他体外或体内系统。
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Cite this Article
Feng, S., Wen, X., Lu, X. Nitropeptide Profiling and Identification Illustrated by Angiotensin II. J. Vis. Exp. (148), e59391, doi:10.3791/59391 (2019).
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