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Nitropeptide Profiling and Identification Illustrated by Angiotensin II
1Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, 2Department of Biological Sciences,University of Notre Dame, 3Department of Urology, Shanghai East Hospital,Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases,University of Notre Dame, 5Harper Cancer Research Institute,University of Notre Dame, 6Tumor Microenvironment and Metastasis Program,Indiana University Melvin and Bren Simon Cancer Center
章
- 00:04標題
- 00:32Nitro-Angiotensin II Desalting
- 01:30Primary Amine Alkylation by Dimethyl Labeling
- 02:37Nitrotyrosine to Aminotyrosine Reduction and Biotinylation, Enrichment, and Detection
- 06:03Results: Representative Mass Spectra Analyses
- 06:59Conclusion
Proteomic profiling of tyrosine-nitrated proteins has been a challenging technique due to the low abundance of the 3-nitrotyrosine modification. Here we describe a novel approach for nitropeptide enrichment and profiling by using Angiotensin II as the model. This method can be extended for other in vitro or in vivo systems.
